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Article

Workflow to Characterize Mutants with Reproductive Defects.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Noble Jennifer A, Palanivelu Ravishankar

Abstract excerpt

Reverse genetics approaches for characterizing phenotypes of mutants in a gene of interest (GOI) require thorough genotyping and phenotypic analysis. However, special challenges are encountered when a GOI is expressed in reproductive tissues: a variety of assays are required to characterize the phenotype and a mutant may show sporophytic and/or gametophytic defects in male and/or female reproductive tissues,...

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PMID: 32529432
DOI: 10.1007/978-1-0716-0672-8_8

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