Article
A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase.
Biocontrol science - 1 Jan 2020
Kubosaki Atsutaka, Kobayashi Naoki, Watanabe Maiko, Yoshinari Tomoya, Takatori Kosuke, Kikuchi Yutaka, Hara-Kudo Yukiko, Terajima Jun, Sugita-Konishi Yoshiko
Abstract excerpt
Aspergillus section Versicolores species, except Aspergillus sydowii, produce a carcinogenic mycotoxin sterigmatocystin (STC). Since these fungi are found in varied environmental milieu including indoor dust and food products, our aim was to develop a sensitive and convenient assay to detect STC producing fungal strains. We made use of a high discrimination DNA polymerase (HiDi DNA polymerase), for single...
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