Article
High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction.
Journal of bioscience and bioengineering - 1 Dec 2006
Suzuki Satoshi, Taketani Hiroko, Kusumoto Ken-Ichi, Kashiwagi Yutaka
Abstract excerpt
High-throughput genotyping of Aspergillus oryzae was achieved using an FTA card for the extraction of a genomic DNA template for polymerase chain reaction from a fungal colony growing on an agar plate. This method was then applied to detect other fungal species from agar slants and food materials. This method offers a convenient tool for the genotyping of filamentous fungi without using an organic solvent or...
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