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Article

The visible skeleton 2.0: phenotyping of cartilage and bone in fixed vertebrate embryos and foetuses based on X-ray microCT.

Development (Cambridge, England) - 29 May 2020

Gabner Simone, Böck Peter, Fink Dieter, Glösmann Martin, Handschuh Stephan

Abstract excerpt

For decades, clearing and staining with Alcian Blue and Alizarin Red has been the gold standard to image vertebrate skeletal development. Here, we present an alternate approach to visualise bone and cartilage based on X-ray microCT imaging, which allows the collection of genuine 3D data of the entire developing skeleton at micron resolution. Our novel protocol is based on ethanol fixation and staining with...

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PMID: 32439754
DOI: 10.1242/dev.187633

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