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Development of a real‐time PCR assay for detection of African swine fever virus with an endogenous internal control

19 Apr 2020

Abstract excerpt

Real-time PCR assays are highly sensitive, specific and rapid techniques for the identification of ASF virus (ASFV) (Section 3.8, OIE Terrestrial Manual, 2019). Although an ASFV p72 gene-based real-time PCR assay (a.k.a. the Zsak assay) (Journal of Clinical Microbiology, 2005, 43, 112) has been widely used for ASFV detection, several more ASFV whole genome sequences have become available in the 15 years since the...

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PMID: 32306531
DOI: 10.1111/tbed.13582

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