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DEN-IM: dengue virus genotyping from amplicon and shotgun metagenomic sequencing.

Microbial genomics - 1 Mar 2020

Mendes Catarina I, Lizarazo Erley, Machado Miguel P, Silva Diogo N, Tami Adriana, Ramirez Mário, Couto Natacha, Rossen John W A, Carriço João A

Abstract excerpt

Dengue virus (DENV) represents a public health threat and economic burden in affected countries. The availability of genomic data is key to understanding viral evolution and dynamics, supporting improved control strategies. Currently, the use of high-throughput sequencing (HTS) technologies, which can be applied both directly to patient samples (shotgun metagenomics) and to PCR-amplified viral sequences (amplicon...

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PMID: 32134380
DOI: 10.1099/mgen.0.000328
PMCID: PMC7200064

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