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Generating Gene Silenced Mutants in Phytophthora sojae.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Zhao Yao, Li Yufei, Qiu Min, Ma Wenbo, Wang Yuanchao

Abstract excerpt

Phytophthora species are notorious pathogens of plants and cause enormous damage to agriculture. In order to understand Phytophthora pathogenesis, gene silencing and knockout methods are important for the investigation of gene functions. Although CRISPR/Cas9-based gene knockout procedures have been developed in Phytophthora sojae and Phytophthora capsici, it may not always be the best choice especially when...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 30182241
DOI: 10.1007/978-1-4939-8724-5_18

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