Article
Evolutionary Adaptation to Generate Mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
de Vries Ronald P, Lubbers Ronnie, Patyshakuliyeva Aleksandrina, Wiebenga Ad, Benoit-Gelber Isabelle
Abstract excerpt
In this chapter we describe a method to generate mutants of filamentous fungi using their genomic plasticity and rapid adaptability to their environment. This method is based on spontaneous mutations occurring in relation to improved growth of fungi on media by repeated inoculation resulting in adaptation of the strain to the condition. The critical aspect of this method is the design of the selective media,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.