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Increased pregnancy losses following serial somatic cell nuclear transfer in goats

17 May 2018

Abstract excerpt

Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of precious transgenic lines or resetting the lifespan of primary transgenic cells for multiple genetic modifications. We successfully produced second-generation cloned goats using donor neonatal fibroblasts from first-generation clones. However, our attempts to produce any third-generation clones failed. SCNT efficiency...

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PMID: 29769162
DOI: 10.1071/rd17323

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