Article
A new method based on SNP of nrDNA-ITS to identify Saccharum spontaneum and its progeny in the genus Saccharum.
PloS one - 1 Jan 2018
Yang Shan, Li Xueting, Huang Fei, Huang Yongji, Liu Xinlong, Wu Jiayun, Wang Qinnan, Deng Zuhu, Chen Rukai, Zhang Muqing
Abstract excerpt
The identification of germplasm resources is an important aspect of sugarcane breeding. The aim of this study was to introduce a new method for identifying Saccharum spontaneum and its progeny. First, we cloned and sequenced nuclear ribosomal DNA internal transcribed spacer (nrDNA-ITS) sequences from 20 Saccharum germplasms. Analysis of these nrDNA-ITS sequences showed a stable mutation at base 89. Primers (FO13,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
