Article
Phenotyping Tumor-Associated Macrophages.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Ian Cumming R, Yu Yen-Rei A
Abstract excerpt
Multi-parametric flow cytometry of tumor-bearing murine nonlymphoid tissue allows for characterization, isolation, and examination of immune cell composition, phenotype, and function. Here we describe an approach to process nonlymphoid tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This panel can be used to phenotype tumor-associated...
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