Article
[Multiplex Genotyping of Allelic Variants of Genes Involved in Metabolizing Antileukemic Drugs].
Molekuliarnaia biologiia - 1 Jan 2000
Fesenko D O, Avdonina M A, Gukasyan L G, Surzhikov S A, Chudinov A V, Zasedatelev A S, Nasedkina T V
Abstract excerpt
A biochip, primer set, and genotyping protocol were developed to simultaneously address 16 single nucleotide polymorphisms in antileukemic drug metabolism genes, including TPMT, ITPA, MTHFR, SLCO1B1, SLC19A1, NR3C1, GRIA1, ASNS, MTRR, and ABCB1. The genotyping procedure included a one-round multiplex polymerase chain reaction (PCR) with simultaneous incorporation of a fluorescent label into the PCR product and...
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