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Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

11 Apr 2018

Abstract excerpt

Abstract The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and...

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PMID: 29643334
DOI: 10.1038/s41467-018-03772-1

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