Article
Variant Linkage Analysis Using de Novo Transcriptome Sequencing Identifies a Conserved Phosphine Resistance Gene in Insects.
Genetics - 1 May 2018
Schlipalius David I, Tuck Andrew G, Jagadeesan Rajeswaran, Nguyen Tam, Kaur Ramandeep, Subramanian Sabtharishi, Barrero Roberto, Nayak Manoj, Ebert Paul R
Abstract excerpt
Next-generation sequencing methods enable identification of the genetic basis of traits in species that have no prior genomic information available. The combination of next-generation sequencing, variant analysis, and linkage is a powerful way of identifying candidate genes for a trait of interest. Here, we used a comparative transcriptomics [RNA sequencing (RNAseq)] and genetic linkage analysis approach to...
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