Article
Tetrad Dissection in Fission Yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Escorcia Wilber, Forsburg Susan L
Abstract excerpt
Tetrad dissection is a powerful tool in yeast genetics that allows the analysis of products of a single meiosis. With just a few tetrads, it is possible to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days. With the help of a micromanipulator, ripe asci are isolated to spots 5 mm apart on a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
