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Article

Development of a novel digital RT-PCR method for detection of human sapovirus in different matrices.

Journal of virological methods - 1 Apr 2018

Varela Miguel F, Monteiro Silvia, Rivadulla Enrique, Santos Ricardo, Romalde Jesús L

Abstract excerpt

A new nanofluidic digital RT-PCR method was developed for sapovirus (SaV) using control material obtained according to standards for enteric viruses. Primers employed amplify a fragment of 112 bp of the polymerase capsid junction, allowing the detection of human genogroups I, II and IV. Analytical validation was performed in clinical, shellfish and environmental water samples. This novel protocol rendered great...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29407209
DOI: 10.1016/j.jviromet.2018.01.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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