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Detection of human sapovirus by real‐time reverse transcription‐polymerase chain reaction

22 Aug 2006

Abstract excerpt

Sapovirus (SaV) is an agent of gastroenteritis for humans and swine, and is divided into five distinct genogroups (GI-GV) based on its capsid gene sequences. Typical methods of SaV detection include electron microscopy (EM), enzyme-linked immunosorbent assay (ELISA), and reverse transcription-polymerase chain reaction (RT-PCR). A novel TaqMan-based real-time RT-PCR assay was developed that is sensitive and has...

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PMID: 16927293
DOI: 10.1002/jmv.20699

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