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Droplet digital RT-PCR method for SARS-CoV-2 variants detection in clinical and wastewater samples

3 Jul 2025

Abstract excerpt

Objective: To establish a sensitive, specific, and precise quantitative detection method for SARS-CoV-2 variants using droplet digital RT-PCR (RT-ddPCR). Methods: Dual primer-probe sets targeting the SARS-CoV-2 nucleocapsid (N) and spike (S) genes were designed. The annealing temperature for RT-ddPCR was optimized using a gradient PCR system. The sensitivity, defined as the limit of detection (LOD), was...

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PMID: 40678046
DOI: 10.3389/fmicb.2025.1635733

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