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Article

CRISPR/Cas9 genome editing in human hematopoietic stem cells.

Nature protocols - 1 Feb 2018

Bak Rasmus O, Dever Daniel P, Porteus Matthew H

Abstract excerpt

Genome editing via homologous recombination (HR) (gene targeting) in human hematopoietic stem cells (HSCs) has the power to reveal gene-function relationships and potentially transform curative hematological gene and cell therapies. However, there are no comprehensive and reproducible protocols for targeting HSCs for HR. Herein, we provide a detailed protocol for the production, enrichment, and in vitro and in...

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Identifiers

PMID: 29370156
DOI: 10.1038/nprot.2017.143
PMCID: PMC5826598

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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