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Article

A straightforward assay for measuring glycogen levels and RpoS.

Journal of microbiological methods - 1 Feb 2018

Iwase Tadayuki, Okai Chiaki, Kamata Yuko, Tajima Akiko, Mizunoe Yoshimitsu

Abstract excerpt

Cellular glycogen levels reflect the activity of RpoS, an important stress-inducible bacterial sigma factor known to regulate several stress-resistance related genes, such as katE, encoding hydroperoxidase II (HPII), and the glg genes, encoding glycogen synthesis enzymes, in Escherichia coli. In this study, a straightforward assay for measuring glycogen levels and RpoS activity was developed combining the ease...

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Identifiers

PMID: 29288674
DOI: 10.1016/j.mimet.2017.12.008

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