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Article

N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.

The New phytologist - 1 May 2018

Zhang Hongtao, Gannon Lucy, Hassall Kirsty L, Deery Michael J, Gibbs Daniel J, Holdsworth Michael J, van der Hoorn Renier A L, Lilley Kathryn S, Theodoulou Frederica L

Abstract excerpt

The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the proteome of etiolated seedlings, we used terminal amine isotopic labelling of substrates with tandem mass tags (TMT-TAILS) for relative quantification of N-terminal...

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PMID: 29168982
DOI: 10.1111/nph.14909
PMCID: PMC5947142

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