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[Study on identification of Dendrobium officinale and related species by bidirectional PCR amplification of mismatched and specific alleles].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Mar 2017

Dong Xiao-Man, Jiang Chao, Yuan Yuan, Zha Liang-Ping, Peng Dai-Yin, Zhao Yu-Yang

Abstract excerpt

Based on rDNA ITS sequences of Dendrobium officinale and the other 69 species of Dendrobium, a pair of dismatched allele-specific diagnostic primers, TPSH-AS1F and TPSH-AS1R were designed to authenticate D. officinale from the other species. Thebidirectional PCR amplification were performed using the diagnostic primers with the total DNAs of the original plants or processing products as a template. When the...

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PMID: 28994532
DOI: 10.19540/j.cnki.cjcmm.20170217.001

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