Article
Allele-specific primers for diagnostic PCR authentication of Dendrobium officinale.
Planta medica - 1 Jun 2003
Ding Xiaoyu, Wang Zhengtao, Zhou Kaiya, Xu Luoshan, Xu Hong, Wang Yiquan
Abstract excerpt
Based on rDNA ITS sequences of D. officinale and the other 37 species of Dendrobium, a pair of allele-specific diagnostic primers, TP-JB01S and TP-JB01X, were designed to authenticate D. officinale from the other species. Before the diagnostic PCR, the primer pair, P1 and P2, for amplifying the whole ITS region was used to validate template DNA and to obtain the appropriate template DNA for the diagnostic PCR....
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