Article
[Allele-specific diagnostic PCR authentication of Dendrobium thyrsiflorum].
Yao xue xue bao = Acta pharmaceutica Sinica - 1 Jan 2007
Ying Yi, Xu Hong, Wang Zheng-Tao
Abstract excerpt
The aim of this study was to establish a diagnostic PCR method for authentication of D. thyrsiflorum Rchb. f. Based on rDNA ITS sequences of the 164 samples from 109 Dendrobium species sequenced and quoted from GenBank, the allele-specific diagnostic primers QH-JB1 and QH-JB2 for authentication were designed. Using the primers, diagnostic PCR was performed with the total DNA of D. thyrsiflorum Rchb. f. and other...
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