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Rapid Identification of Rust Resistance Genes Through Cultivar-Specific De Novo Chromosome Assemblies.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Thind Anupriya K, Wicker Thomas, Krattinger Simon G

Abstract excerpt

"Map-based cloning" is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert...

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PMID: 28856656
DOI: 10.1007/978-1-4939-7249-4_21

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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