Article
Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat.
Genetics - 1 Jun 2003
Huang Li, Brooks Steven A, Li Wanlong, Fellers John P, Trick Harold N, Gill Bikram S
Abstract excerpt
We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a gene-rich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans...
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