Article
Cryptosporidium in fish: alternative sequencing approaches and analyses at multiple loci to resolve mixed infections.
Parasitology - 1 Nov 2017
Paparini Andrea, Yang Rongchang, Chen Linda, Tong Kaising, Gibson-Kueh Susan, Lymbery Alan, Ryan Una M
Abstract excerpt
Currently, the systematics, biology and epidemiology of piscine Cryptosporidium species are poorly understood. Here, we compared Sanger ‒ and next-generation ‒ sequencing (NGS), of piscine Cryptosporidium, at the 18S rRNA and actin genes. The hosts comprised 11 ornamental fish species, spanning four orders and eight families. The objectives were: to (i) confirm the rich genetic diversity of the parasite and the...
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