Temporal dynamics of bacterial microbiota in the human oral cavity determined using an in situ model of dental biofilms

Abstract excerpt

Abstract Numerous studies on oral biofilms have been performed in vitro , although it is difficult to mimic the oral environment. Here we used an in situ model to conduct a quantitative analysis and comprehensive identification of bacterial communities over time by performing deep sequencing of 16S rRNA genes. We show here that the number of viable bacteria in supragingival biofilms increased in two steps. Using...

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