Article
Improved high sensitivity screen for Huntington disease using a one-step triplet-primed PCR and melting curve assay.
PloS one - 1 Jan 2017
Zhao Mingjue, Cheah Felicia S H, Chen Min, Lee Caroline G, Law Hai-Yang, Chong Samuel S
Abstract excerpt
Molecular diagnosis of Huntington disease (HD) is currently performed by fluorescent repeat-flanking or triplet-primed PCR (TP-PCR) with capillary electrophoresis (CE). However, CE requires multiple post-PCR steps and may result in high cost in high-throughput settings. We previously described a cost-effective single-step molecular screening strategy employing the use of melting curve analysis (MCA). However,...
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