Article
Serotyping dengue virus with isothermal amplification and a portable sequencer.
Scientific reports - 14 Jun 2017
Yamagishi Junya, Runtuwene Lucky R, Hayashida Kyoko, Mongan Arthur E, Thi Lan Anh Nguyen, Thuy Linh Nguyen, Nhat Cam Nguyen, Limkittikul Kriengsak, Sirivichayakul Chukiat, Sathirapongsasuti Nuankanya, Frith Martin, Makalowski Wojciech, Eshita Yuki, Sugano Sumio, Suzuki Yutaka
Abstract excerpt
The recent development of a nanopore-type portable DNA sequencer has changed the way we think about DNA sequencing. We can perform sequencing directly in the field, where we collect the samples. Here, we report the development of a novel method to detect and genotype tropical disease pathogens, using dengue fever as a model. By combining the sequencer with isothermal amplification that only requires a water bath,...
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