Article
qPCR for the detection of foodborne Trypanosoma cruzi.
Parasitology international - 1 Oct 2017
de Souza Godoi Poliana Alves, Piechnik Claudio Adriano, de Oliveira Ana Caroline, Sfeir Michelle Zibetti, de Souza Emanuel Maltempi, Rogez Hervé, Thomaz Soccol Vanete
Abstract excerpt
Here we presented a potential real-time PCR (qPCR) method with public health importance and relevance for detection of Trypanosoma cruzi in açai pulp. There is not a current process to identify T. cruzi in açai, that ensures innocuity of this food concerning oral transmission. First, six new primers were designed using the DNA sequences of T. cruzi y152 and Emerald strains obtained from GenBank. For primers...
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