Article
Efficient protein production inspired by how spiders make silk.
Nature communications - 23 May 2017
Kronqvist Nina, Sarr Médoune, Lindqvist Anton, Nordling Kerstin, Otikovs Martins, Venturi Luca, Pioselli Barbara, Purhonen Pasi, Landreh Michael, Biverstål Henrik, Toleikis Zigmantas, Sjöberg Lisa, Robinson Carol V, Pelizzi Nicola, Jörnvall Hans, Hebert Hans, Jaudzems Kristaps, Curstedt Tore, Rising Anna, Johansson Jan
Abstract excerpt
Membrane proteins are targets of most available pharmaceuticals, but they are difficult to produce recombinantly, like many other aggregation-prone proteins. Spiders can produce silk proteins at huge concentrations by sequestering their aggregation-prone regions in micellar structures, where the very soluble N-terminal domain (NT) forms the shell. We hypothesize that fusion to NT could similarly solubilize...
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