Replacement of cysteine-107 of Saccharomyces cerevisiae iso-1-cytochrome c with threonine: improved stability of the mutant protein.

doi:10.1093/protein/1.2.95

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Article

Replacement of cysteine-107 of Saccharomyces cerevisiae iso-1-cytochrome c with threonine: improved stability of the mutant protein.

Protein engineering - 1 Jan 2000

Cutler R L, Pielak G J, Mauk A G, Smith M

Abstract excerpt

Site-directed mutagenesis has been used to change the codon for cysteine-107 of Saccharomyces cerevisiae iso-1-cytochrome c to a threonine codon. The resulting protein is active in vivo, is methylated as in the wild-type protein and has optical properties indistinguishable from those of the wild-...

Topics

Amino Acid Sequence
Cytochrome c Group
Cytochromes c
Electrochemistry
Molecular Sequence Data
Mutation
Protein Engineering
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins

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