Article
Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes.
Genome - 1 Feb 2017
Du Pei, Zhuang Lifang, Wang Yanzhi, Yuan Li, Wang Qing, Wang Danrui, Dawadondup, Tan Lijun, Shen Jian, Xu Haibin, Zhao Han, Chu Chenggen, Qi Zengjun
Abstract excerpt
In comparison with general FISH for preparing probes in terms of time and cost, synthesized oligonucleotide (oligo hereafter) probes for FISH have many advantages such as ease of design, synthesis, and labeling. Low cost and high sensitivity and resolution of oligo probes greatly simplify the FISH procedure as a simple, fast, and efficient method of chromosome identification. In this study, we developed new oligo...
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