Article
A metagenomics study for the identification of respiratory viruses in mixed clinical specimens: an application of the iterative mapping approach.
Archives of virology - 1 Jul 2017
Gong Yu-Nong, Yang Shu-Li, Chen Guang-Wu, Chen Yu-Wen, Huang Yhu-Chering, Ning Hsiao-Chen, Tsao Kuo-Chien
Abstract excerpt
Metagenomic approaches to detect viral genomes and variants in clinical samples have various challenges, including low viral titers and bacterial and human genome contamination. To address these limitations, we examined a next-generation sequencing (NGS) and iterative mapping approach for virus detection in clinical samples. We analyzed 40 clinical specimens from hospitalized children diagnosed with acute...
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