Article
Isolation of mesenchymal stromal/stem cells from cryopreserved umbilical cord blood cells.
Journal of clinical and experimental hematopathology : JCEH - 5 Jul 2017
Fujii Sumie, Miura Yasuo, Iwasa Masaki, Yoshioka Satoshi, Fujishiro Aya, Sugino Noriko, Kaneko Hitomi, Nakagawa Yoko, Hirai Hideyo, Takaori-Kondo Akifumi, Ichinohe Tatsuo, Maekawa Taira
Abstract excerpt
Umbilical cord blood (UCB) has advantages over other tissues because it can be obtained without an invasive procedure and complex processing. We explored the availability of cryopreserved UCB cells as a source of mesenchymal stromal/stem cells (MSCs). MSCs were successfully isolated from six of 30 UCB units (median volume, 34.0 mL; median nucleated cell number, 4.4×108) that were processed and cryopreserved using...
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