Article
Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq - ryhB encodes the regulatory RNA RyhB and a peptide, RyhP.
BMC genomics - 28 Feb 2017
Neuhaus Klaus, Landstorfer Richard, Simon Svenja, Schober Steffen, Wright Patrick R, Smith Cameron, Backofen Rolf, Wecko Romy, Keim Daniel A, Scherer Siegfried
Abstract excerpt
BACKGROUND: While NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHEC. A high ratio of ribosomal footprints per transcript (ribosomal coverage value, RCV) is expected to indicate a...
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