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Article

Superior Multiplexing Capacity of PlexPrimers Enables Sensitive and Specific Detection of SNPs and Clustered Mutations in qPCR.

PloS one - 1 Jan 2017

Tan Lit Yeen, Walker Samantha Michelle, Lonergan Tina, Lima Nicole Elizabeth, Todd Alison Velyian, Mokany Elisa

Abstract excerpt

BACKGROUND: Whilst qPCR provides an extremely powerful tool for genetic analysis, some applications such as multiplexing variant alleles (eg SNPs, point mutations or deletions), remain challenging using current primer/probe systems. The novel design features of PlexPrimers allow sensitive, multiplexed analysis of variant alleles even when these are tightly clustered. METHOD: PlexPrimers were combined with...

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Identifiers

PMID: 28114309
DOI: 10.1371/journal.pone.0170087
PMCID: PMC5256879

Genes

EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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