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Article

Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform.

BMC microbiology - 21 Jan 2017

Castelino Madhura, Eyre Stephen, Moat John, Fox Graeme, Martin Paul, Ho Pauline, Upton Mathew, Barton Anne

Abstract excerpt

BACKGROUND: The composition of the skin microbiome is predicted to play a role in the development of conditions such as atopic eczema and psoriasis. 16S rRNA gene sequencing allows the investigation of bacterial microbiota. A significant challenge in this field is development of cost effective high throughput methodologies for the robust interrogation of the skin microbiota, where biomass is low. Here we describe...

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Identifiers

PMID: 28109256
DOI: 10.1186/s12866-017-0927-4
PMCID: PMC5251215

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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