High-throughput whole genome sequencing of <i>Porcine reproductive and respiratory syndrome virus</i> from cell culture materials and clinical specimens using next-generation sequencing technology

Abstract excerpt

Next-generation sequencing (NGS) technologies have increasingly played crucial roles in biological and medical research, but are not yet in routine use in veterinary diagnostic laboratories. We developed and applied a procedure for high-throughput RNA sequencing of Porcine reproductive and respiratory syndrome virus (PRRSV) from cell culture-derived isolates and clinical specimens. Ten PRRSV isolates with known...

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