Nested PCR targeting intergenic spacer (IGS) in genotyping of Giardia duodenalis isolated from symptomatic and asymptomatic infected Egyptian school children.

doi:10.1007/s00436-016-5347-0

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Article

Nested PCR targeting intergenic spacer (IGS) in genotyping of Giardia duodenalis isolated from symptomatic and asymptomatic infected Egyptian school children.

Parasitology research - 1 Feb 2017

Hussein Eman M, Ismail Ola A, Mokhtar Amira B, Mohamed Samer E, Saad Rania M

Abstract excerpt

Distinct sequences of Giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. However, sequences analysis is time consuming, expensive, and needs many manual operations. Nested PCR targeting intergenic spacer (IGS) region was applied successfully to genotype G. duodenalis. This study aimed to identify the prevalence of G. duodenalis assemblages among...

Topics

Adolescent
Animals
Child
Child, Preschool
Coinfection
Diarrhea
Egypt
Feces
Female
Genotype
Giardia lamblia
Giardiasis
Humans
Male
Polymerase Chain Reaction
Prevalence

Source

Open in PubMed

Identifiers

PMID: 27975120
DOI: 10.1007/s00436-016-5347-0

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