Article
Application of Single-Molecule Amplification and Resequencing Technology for Broad Surveillance of Plasma Mutations in Patients with Advanced Lung Adenocarcinoma.
The Journal of molecular diagnostics : JMD - 1 Jan 2017
Wang Zheng, Cheng Gang, Han Xiaohong, Mu Xinlin, Zhang Yuhui, Cui Di, Liu Chang, Zhang Li, Fan Zaiwen, Ma Lingyun, Yang Li, Di Jing, Cram David S, Shi Yuankai, Liu Dongge
Abstract excerpt
Liquid biopsy to access the circulating tumor DNA is a promising surrogate for invasive tumor genotyping. We designed a multiplex assay based on circulating single-molecule amplification and resequencing technology (cSMART) to simultaneously detect and quantitate hot spot EGFR, KRAS, BRAF, ERBB2, and ALK plasma DNA variants in 103 patients with advanced lung adenocarcinoma. In validation studies using an...
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