Article
Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum.
Scientific reports - 18 Nov 2016
Zhao Pei, Wang Ke, Lin Zhishan, Zhang Wei, Du Lipu, Zhang Yunlong, Ye Xingguo
Abstract excerpt
Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an important role in T-DNA transport and integration. In this study, a TaVIP2 gene was cloned from common wheat. Southern blot and allele-specific polymerase chain reaction (AS-PCR) combined with an online...
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