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Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay

10 Nov 2016

Abstract excerpt

ABSTRACT Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia . Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing analysis of the PCR product to differentiate Leishmania spp. Here we describe a novel real-time...

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PMID: 27847378
DOI: 10.1128/jcm.01764-16

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