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Design and Validation of Real-Time PCR: Quantitative Diagnosis of Common Leishmania Species in Iran.

1 Jul 2016

Abstract excerpt

OBJECTIVE: Design and validation of Real-time PCR on the protected gene region ITS2 to quantify the parasite load in common leishmania (L) species. MATERIALS AND METHODS: Probe and primer were designed from the ITS2 region between the rRNA genes with minimum gene variation in three common leishmania species followed by a Real-time PCR using the Taq man probe method in the form of absolute quantification. A series...

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PMID: 27362244

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