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Identification of Leishmania spp. by Molecular Amplification and DNA Sequencing Analysis of a Fragment of rRNA Internal Transcribed Spacer 2

14 Jul 2011

Abstract excerpt

Isoenzyme analysis of cultured parasites is the conventional approach for Leishmania species identification. Molecular approaches have the potential to be more sensitive and rapid. We designed PCR generic primers to amplify a segment of the rRNA internal transcribed spacer 2 (ITS2) from multiple Leishmania species. To validate the selected ITS2 fragment, we tested clinical specimens and compared the species...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 21752983
DOI: 10.1128/jcm.01177-11

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