Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score.

doi:10.18632/oncotarget.13066

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Article

Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score.

Oncotarget - 13 Dec 2016

Tekin Nilgun, Omidvar Nader, Morris Tim Peter, Conget Paulette, Bruna Flavia, Timar Botond, Gagyi Eva, Basak Ranjan, Naik Omkar, Auewarakul Chirayu, Sritana Narongrit, Levy Debora, Cerci Juliano Julio, Bydlowski Sergio Paulo, Pereira Juliana, Dimamay Mark Pierre, Natividad Filipinas, Chung June-Key, Belder Nevin, Kuzu Isinsu, Paez Diana, Dondi Maurizio, Carr Robert, Ozdag Hilal, Padua Rose Ann

Abstract excerpt

As a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to validate the previously reported 6-gene (LMO2, BCL6, FN1, CCND2, SCYA3 and BCL2) model as predictor of prognosis in...

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Identifiers

PMID: 27825111
DOI: 10.18632/oncotarget.13066
PMCID: PMC5347772

Genes

BCL2
BCL6
CCND2
EFS
LMO2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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