Article
Orthogonal design in the optimization of a start codon targeted (SCoT) PCR system in Roegneria kamoji Ohwi.
Genetics and molecular research : GMR - 24 Oct 2016
Zeng B, Yan H D, Huang L K, Wang Y C, Wu J H, Huang X, Zhang A L, Wang C R, Mu Q
Abstract excerpt
Roegneria kamoji Ohwi is an excellent forage grass due to its high feeding value and high resistance to some biotic and abiotic stresses. However, the start codon targeted (SCoT) polymorphism has not been conducted on R. kamoji. In this study, an orthogonal L16 (45) design was employed to investigate the effects of five factors (Mg2+, dNTPs, Taq DNA polymerase, primer, and template DNA) on the polymerase chain...
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