Direct detection ofmecA,blaSHV, blaCTX-M,blaTEMandblaOXAgenes from positive blood culture bottles by multiplex-touchdown PCR assay

Abstract excerpt

Methicillin‐resistant staphylococci (MRS) and ESBL(Extended‐Spectrum β‐Lactamase)‐producing bacteria are the most important resistant pathogens in sepsis. In this study, a new multiplex‐touchdown PCR method (MT‐PCR) was developed to detect rapidly and simultaneously the presence of mecA, blaSHV, blaCTX‐M, blaTEM and blaOXA genes from positive blood culture bottles. The technique showed a sensitivity of 103 CFU...

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