Article
Translation, modification and cellular distribution of two AC4 variants of African cassava mosaic virus in yeast and their pathogenic potential in plants.
Virology - 1 Nov 2016
Hipp Katharina, Rau Peter, Schäfer Benjamin, Pfannstiel Jens, Jeske Holger
Abstract excerpt
Plant infecting geminiviruses encode a small (A)C4 protein within the open reading frame of the replication-initiator protein. In African cassava mosaic virus, two in-frame start codons may be used for the translation of a longer and a shorter AC4 variant. Both were fused to green fluorescent protein or glutathione-S-transferase genes and expressed in fission yeast. The longer variant accumulated in discrete...
Topics
- Amino Acid Sequence
- Begomovirus
- Gene Expression
- Genes, Reporter
- Genome, Viral
- Microscopy, Confocal
- Phenotype
- Plant Diseases
- Protein Biosynthesis
- Protein Transport
- Recombinant Fusion Proteins
- Schizosaccharomyces
- Viral Proteins
- Yeasts